Spectral Flow Cytometry
Sony ID7000 Spectral Flow Cytometer
The Sony ID7000 "Sirius" Spectral Flow Cytometer is a cutting-edge spectral flow cytometer analyzer equipped with 5 lasers and 147 fluorescence photodetectors, giving the instrument the ability to detect more than 40 fluorochromes in a single sample. The full spectrum detection capabilities of the instrument permit measurement of virtually any excitable fluorochrome without dedicated filter sets. In addition, by measuring spectral signatures of each fluourochrome, the ID7000 can detect the presence of nearly overlapping dyes. Furthermore, autofluorescence can be treated as a unique cellular parameter and subtracted from total fluorescence, reducing background and increasing resolution.
At the heart of the ID7000 is the best-in-class auto-loader capable of true hands-free sample
acquisition from a variety of plate types, including both 96 and 384-well formats, and a multi-tube rack. Temperature control and sample mixing are integrated into the auto-loader and can be programmed and tailored uniquely to each experiment. Intuitive ID7000 Software simplifies the workflow for setting up a high parameter experiment, collecting data, and
accurately performing spectral unmixing.
Excitation: (all lasers are spatially separated)
- 355 nm
- 405 nm
- 488 nm
- 561 nm
- 637 nm
Detection:
- 147 fluorescence detectors (multichannel and single channel PMTs) spanning the visible spectrum
- Light is partitioned by wavelength for spectral detection using custom diffraction grating technology
- Detection is apportioned per laser as described below:
- 355 nm laser: 35 independent detectors (three single-channel PMTs + one 32 channel PMT)
- 405 nm laser: 35 independent detectors (three single-channel PMTs + one 32 channel PMT)
- 488 nm laser: 32 independent detectors (32-channel PMT)
- 561 nm laser: 26 independent detectors (multichannel PMT)
- 637 nm laser 19 independent detectors (multichannel PMT)
- Forward and side scatter (488 nm)