Stem Cell Core
The Stem Cell Core Facility is a multi-center facility at Columbia University Irving Medical Center, created to help users take advantage of up-to-date resources in the stem cell field in a timely and cost-efficient way.
The Columbia Stem Cell Core Facility has two primary aims:
- To give our users access to quality-controlled pluripotent stem cells and stem cell-derivatives prepared using standardized approaches
- To offer genome editing services for the generation of custom-designed cell lines
The Stem Cell Core Facility also offers training sessions and informal interactions between scientists from multiple laboratories working on different stem cell models. Some reagents commonly used in stem cell labs, as mTeSR1 media and irradiated mouse embryonic fibroblasts, are also available at discounted prices for our users.
We are connected to Stem Cell COREdinates, a consortium of pluripotent stem cell-focused core facilities, which allows us to take advantage of the sharing of the most updated and efficient protocols, expertise, and reagents.
The Cell Production section of the Stem Cell Core Facility offers to its users maintenance and supply of human ESC/iPSC; derivation and characterization of hiPSC from fibroblasts or blood; and differentiation of human pluripotent stem cells into functional, mature, highly purified progeniesto (including neuronal, cardiac, retinal pigment epithelium, hepatic, pancreatic & skeletal muscle.)
The Gene Editing section of the Stem Cell Core Facility provides various services, including insertion of transgene and targeted single nucleotide modifications.
Stem Cell Equipment
Some equipment, including the picking hood, the Lonza Nucleofector 4D and the Life Technologies Neon, is available to users for rental. Please contact us for more information.
Patel A, Diaz AG, Moore JC, Sirabella D & Corneo B. Establishment and characterization of two iPSC lines derived from healthy controls. Stem Cell Research, 2020 Jul 25;47:101926.
Garcia-Diaz A., Efe G., Kabra K, Patel A, Lowry E.R., Shneider N., Corneo B., Wichterle H. Standardized reporter systems for purification and imaging of human pluripotent stem cell-derived motor neurons and other cholinergic cells. Neuroscience, 2020 Jun 30:S0306-4522(20)30404-8.
Liu J, Taylor R.L., Baines R.A., Swanton L., Freeman S., Corneo B., Patel A., Marmorstein A., Knudsen T., Black G.C., Manson F. Small molecules restore bestrophin-1 expression and function of both dominant and recessive bestrophinopathies in patient-derived RPE. IOVS. 2020 May 11;61(5):28. PMID: 32421148
Riera M, Patel A, Burés-Jelstrup A, Corcostegui B, Chang S, Pomares E, Corneo B, Sparrow JR. Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations. Stem Cell Res. 2019 Apr;36:101389. PMID: 30798147
Riera M, Patel A, Corcostegui B, Chang S, Sparrow JR, Pomares E, Corneo B. Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations. Stem Cell Res. 2019; 35:101385. PMID: 30685614
Riera M, Patel A, Corcostegui B, Chang S, Corneo B, Sparrow JR, Pomares E. Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene. Stem Cell Res. 2019; 35:101386. PMID: 30685615
Baulier E, Garcia Diaz A, Corneo B, Farber DB. Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143. Stem Cell Res. 2018; 33:274-277. PMID: 30513407
Di Baldassarre A, D'Amico MA, Izzicupo P, Gaggi G, Guarnieri S, Mariggiò MA, Antonucci I, Corneo B, Sirabella D, Stuppia L, Ghinassi B. Cardiomyocytes Derived from Human CardiopoieticAmniotic Fluids. Scientific reports. 2018 Aug 13;8(1):12028. doi: 10.1038/s41598-018-30537-z. PMID: 30104705. PMCID: PMC6089907.
Barbara Corneo, PhD
Associate Professor; Director, Columbia Stem Cell Core Facility
Barbara manages the daily activity of Columbia's Stem Cell Core Facility, bringing her knowledge of stem cell research and enthusiasm for collaborating and teaching to the Columbia stem cell community as well as external users. Barbara grew up in Italy and earned a PhD in Immunology in Paris, France, working on severe combined immunodeficiency and V(D)J recombination. She then moved to the United States where she continued working on the effects that mutations in the Rag1 & 2 genes cause on the immune system. Barbara then joined Dr. Gordon Keller's lab at Mount Sinai, where she learned the basis of hESC biology and worked on endoderm differentiation and specification toward hepatic and pancreatic progenitors. She then joined the Neural Stem Cell Institute, directed by Dr. Sally Temple, to learn more about the nervous system. She worked on iPSC derivation from human ocular tissues and differentiation of hESC and hiPSC into retinal pigment epithelium (RPE). Barbara now directs the Stem Cell Core Facility, to provide support and to offer her expertise and help to any user interested in learning more about adult stem cells, embryonic stem cells, induced pluripotent stem cells, and their differentiated progenies.
Dario Sirabella, PhD
Associate Research Scientist
Dario has a longstanding experience with stem cells. During his PhD at the University of Rome, Italy, he extensively studied skeletal muscle-derived stem cells and fetal vessel-associated stem cells, with particular focus on multipotency and differentiation into multiple mesodermal cell types. During his postdoctoral work at Mount Sinai School of Medicine, NY, he investigated specific molecular mechanisms involved in the signaling cascade that controls myogenic differentiation. Later, he became interested in pluripotency and started working on reprogramming skeletal muscle stem cells and in their transdifferentiation into cardiac cells.
Dario joined the Columbia's Stem Cell Core Facility in 2012 as head of the cardiac section, a key resource for providing standardized preparations of human pluripotent stem cells-derived cardiac lineages. He is now also responsible of training, reprogramming and on the differentiation of hiPSC/hESC into motor neurons and skeletal muscle.
Achchhe Patel, PhD
Associate Research Scientist
Achchhe received his PhD from the University of Delhi, India. He worked as a postdoctoral researcher at Wake Forest University School of Medicine, NC, to understand host-pathogen interactions of the intracellular parasite Chlamydia trachomatis. He entered the field of stem cell research by joining the laboratory of Dr. Ihor Lemischka at the Icahn School of Medicine at Mount Sinai, New York, and was involved in the identification of gene networks in insulin resistance using patient-derived iPSCs. He worked in collaboration with Dr. Sunita D'Souza (now Director of the Center for Modeling Pediatric Diseases at St. Jude’s Children’s Research Hospital) in the Mount Sinai Stem Cell Core Facility, where he was trained in the generation of iPSCs and development of protocols for differentiation and characterization of skeletal muscle cells and adipocytes. Achchhe joined the Columbia's Stem Cell Core Facility in the summer of 2016. He is in charge of diverse services, including training, reprogramming and differentiation of motor neurons, cortical neurons and brain organoids, hepatocytes, pancreatic progenitors, and skeletal muscle from hPSC. More recently, Achchhe became the head of the stem cell engineering section of the Stem Cell Core Facility.