Barbara Corneo, PhD

Profile Headshot

Overview

Academic Appointments

  • Assistant Professor of Rehabilitation and Regenerative Medicine at CUMC

Administrative Titles

  • Director, Columbia Stem Cell Core Facility

Barbara manages the daily activity of the Columbia Stem Cell Core Facility, bringing her knowledge in stem cell research and enthusiasm for collaborating and teaching to serve the Columbia Stem Cell Community as well as external users. Barbara grew up in Italy and got a PhD in Immunology in Paris, France, working on severe combined immunodeficiency and V(D)J recombination. She then moved to the United States where she continued working on the effects that mutations in the Rag1 & 2 genes cause on the immune system. Barbara then joined Dr. Gordon Keller lab in Mount Sinai, where she learned the basis of hESC biology and worked on endoderm differentiation and specification toward hepatic and pancreatic progenitors. She then joined the Neural Stem Cell Institute, directed by Dr. Sally Temple, to learn more about the nervous system. She worked on iPSC derivation from human ocular tissues and differentiation of hESC and hiPSC into retinal pigment epithelium (RPE). Barbara now directs the Columbia Stem Cell Core Facility, to provide support and to offer her expertise and help to any user interested in learning more about adult stem cells, embryonic stem cells, induced pluripotent stem cells and their differentiated progenies.

Languages

  • Italian
  • French

Gender

  • Female

Credentials & Experience

Education & Training

  • PhD, Immunology, University of Paris VI Rene Descartes

Research

Don't stop 'til you get enough

The Stem Cell Core Facility is a multi-center facility at Columbia University Medical Center, created to help users take advantage of up-to-date resources in the stem cell field in a timely and cost-efficient way.

The Columbia Stem Cell Core Facility has two primary aims:

To give our users access to quality-controlled pluripotent stem cells and stem cell-derivatives prepared using standardized approaches

To offer genome editing services for the generation of custom-designed cell lines

We are connected to Stem Cell COREdinates, a consortium of pluripotent stem cells-focused core facilities, which allows us to take advantage of the sharing of the most updated and efficient protocols, expertise, and reagents.

Research Interests

  • Stem Cells, Disease models

Grants

COLLABORATIVE EFFORT IN STEM CELL BIOLOGY (Private)

Jul 1 2010 - Jun 30 2014

Selected Publications

1. Saini JS, Corneo B, Miller JD, Kiehl TR, Wang Q, Boles NC, Blenkinsop TA, Stern JH, Temple S. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration. Cell stem cell. 2017; 20(5):635-647.e7. NIHMSID: NIHMS845902 PubMed [journal] PMID: 28132833, PMCID: PMC5419856

2. Miyagishima KJ*, Wan Q*, Corneo B*, Sharma R, Lotfi MR, Boles NC, Hua F, Maminishkis A, Zhang C, Blenkinsop T, Khristov V, Jha BS, Memon OS, D'Souza S, Temple S, Miller SS, Bharti K. In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications. Stem cells translational medicine. 2016; 5(11):1562-1574. PubMed [journal] PMID: 27400791, PMCID: PMC5070511. *=co-first authors

3. Maruotti J, Sripathi SR, Bharti K, Fuller J, Wahlin KJ, Ranganathan V, Sluch VM, Berlinicke CA, Davis J, Kim C, Zhao L, Wan J, Qian J, Corneo B, Temple S, Dubey R, Olenyuk BZ, Bhutto I, Lutty GA, Zack DJ. Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells. Proceedings of the National Academy of Sciences of the United States of America. 2015; 112(35):10950-5. PubMed [journal] PMID: 26269569, PMCID: PMC4568212

4. Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima KJ, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K. A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Stem cells translational medicine. 2014; 3(8):911-22. PubMed [journal] PMID: 24873859, PMCID: PMC4116245

5. van de Leemput J, Boles NC, Kiehl TR, Corneo B, Lederman P, Menon V, Lee C, Martinez RA, Levi BP, Thompson CL, Yao S, Kaykas A, Temple S, Fasano CA. CORTECON: a temporal transcriptome analysis of in vitro human cerebral cortex development from human embryonic stem cells. Neuron. 2014; 83(1):51-68. PubMed [journal] PMID: 24991954

6. Boles NC, Hirsch SE, Le S, Corneo B, Najm F, Minotti AP, Wang Q, Lotz S, Tesar PJ, Fasano CA. NPTX1 regulates neural lineage specification from human pluripotent stem cells. Cell reports. 2014; 6(4):724-36. PubMed [journal] PMID: 24529709

7. Stanzel BV, Liu Z, Somboonthanakij S, Wongsawad W, Brinken R, Eter N, Corneo B, Holz FG, Temple S, Stern JH, Blenkinsop TA. Human RPE stem cells grown into polarized RPE monolayers on a polyester matrix are maintained after grafting into rabbit subretinal space. Stem Cell Reports. 2014; 2(1):64-77. PubMed [journal] PMID: 24511471, PMCID: PMC3916756

8. Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton DN, Bissig KD, Kalir T, Jacobs A, Evans T, Evans MJ, Gouon-Evans V. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell. 2013; 12(6):748-60. NIHMSID: NIHMS475561 PubMed [journal] PMID: 23746980, PMCID: PMC3922205

9. Lotz S, Goderie S, Tokas N, Hirsch SE, Ahmad F, Corneo B, Le S, Banerjee A, Kane RS, Stern JH, Temple S, Fasano CA. Sustained levels of FGF2 maintain undifferentiated stem cell cultures with biweekly feeding. PloS one. 2013; 8(2):e56289. PubMed [journal] PMID: 23437109, PMCID: PMC3577833

10. Blenkinsop TA*, Corneo B*, Temple S, Stern JH. Ophthalmologic stem cell transplantation therapies. Regenerative Medicine. 2012; 7(6 Suppl):32-9. NIHMSID: NIHMS434235 PubMed [journal] PMID: 23210809, PMCID: PMC3582380

11. Salero E, Blenkinsop TA, Corneo B, Harris A, Rabin D, Stern JH, Temple S. Adult human RPE can be activated into a multipotent stem cell that produces mesenchymal derivatives. Cell Stem Cell. 2012; 10(1):88-95. PubMed [journal] PMID: 22226358

12. Nostro MC, Sarangi F, Ogawa S, Holtzinger A, Corneo B, Li X, Micallef SJ, Park IH, Basford C, Wheeler MB, Daley GQ, Elefanty AG, Stanley EG, Keller G. Stage-specific signaling through TGFβ family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells. Development (Cambridge, England). 2011; 138(5):861-71. PubMed [journal] PMID: 21270052, PMCID: PMC3035090

13. Corneo B, Temple S. Sense and serendipity aid RPE generation. Cell Stem Cell. 2009; 5(4):347-8. PubMed [journal] PMID: 19796611

14. Nostro MC, Sarangi F, Ogawa S, Holtzinger A, Corneo B, Li X, Micallef SJ, Park IH, Basford C, Wheeler MB, Daley GQ, Elefanty AG, Stanley EG, Keller G. Pancreatic differentiation Cambridge (MA): Harvard Stem Cell Institute; 2008. Chapter pancreatic differentiation PubMed [book] PMID: 23658992

15. Corneo B*, Wendland RL*, Deriano L*, Cui X, Klein IA, Wong SY, Arnal S, Holub AJ, Weller GR, Pancake BA, Shah S, Brandt VL, Meek K, Roth DB. Rag mutations reveal robust alternative end joining. Nature. 2007; 449(7161):483-6. PubMed [journal] PMID: 17898768

16. Corneo B, Benmerah A, Villartay JP. A short peptide at the C terminus is responsible for the nuclear localization of RAG2. European Journal of Immunology. 2002; 32(7):2068-73. PubMed [journal] PMID: 12115628

17. Corneo B*, Moshous D*, Güngör T, Wulffraat N, Philippet P, Le Deist FL, Fischer A, de Villartay JP. Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome. Blood. 2001; 97(9):2772-6. PubMed [journal] PMID: 11313270

18. Moshous D, Callebaut I, de Chasseval R, Corneo B, Cavazzana-Calvo M, Le Deist F, Tezcan I, Sanal O, Bertrand Y, Philippe N, Fischer A, de Villartay JP. Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency. Cell. 2001; 105(2):177-86. PubMed [journal] PMID: 11336668

19. Corneo B, Moshous D, Callebaut I, de Chasseval R, Fischer A, de Villartay JP. Three-dimensional clustering of human RAG2 gene mutations in severe combined immune deficiency. The Journal of Biological Chemistry. 2000; 275(17):12672-5. PubMed [journal] PMID: 10777560

20. Strazzabosco M, Corneo B, Iemmolo RM, Menin C, Gerunda G, Bonaldi L, Merenda R, Neri D, Poletti A, Montagna M, Del Mistro A, Faccioli AM, D'Andrea E. Epstein-Barr virus-associated post-transplant lympho-proliferative disease of donor origin in liver transplant recipients. Journal of Hepatology. 1997; 26(4):926-34. PubMed [journal] PMID: 9126809

21. Menin C, Ometto L, Veronesi A, Roncella S, Corneo B, Montagna M, Coppola V, Veronese ML, Indraccolo S, Amadori A, De Rossi A, Ferrarini M, Chieco-Bianchi L, D'Andrea E. Analysis of Epstein-Barr virus (EBV) type and variant in spontaneous lymphoblastoid cells and Hu-SCID mouse tumours. Molecular and Cellular Probes. 1996; 10(6):453-61. PubMed [journal] PMID: 9025083

22. Menin C, Indraccolo S, Montagna M, Corneo B, Bonaldi L, Leib-Mösch C, Chieco-Bianchi L, D'Andrea E. Identification of a human endogenous LTR-like sequence using HIV-1 LTR specific primers. Molecular and Cellular Probes. 1996; 10(6):443-51. PubMed [journal] PMID: 9025082

23. Montagna M, Santacatterina M, Corneo B, Menin C, Serova O, Lenoir GM, Chieco-Bianchi L, D'Andrea E. Identification of seven new BRCA1 germline mutations in Italian breast and breast/ovarian cancer families. Cancer Research. 1996; 56(23):5466-9. PubMed [journal] PMID: 8968102

24. Menin C, Ometto L, Veronesi A, Montagna M, Coppola V, Veronese ML, Indraccolo S, Bruni L, Corneo B, Amadori A. Dominance of a single Epstein-Barr virus strain in SCID-mouse tumors induced by injection of peripheral blood mononuclear cells from healthy human donors. Virus Research. 1995; 36(2-3):215-31. PubMed [journal] PMID: 7653100