The Flow Cytometry Specialist (Staff Associate) will support all technical activities in the Columbia Stem Cell Initiative (CSCI) Flow Cytometry core facility. CSCI Flow Cytometry is a shared resource providing labs across all Columbia University campuses access to high-end, well maintained flow cytometry, cell sorting, imaging cytometry, and other support instrumentation along with related support services including instrument training, assisted instrument operation, and experimental design, troubleshooting, and data analysis support.
Cytometry instrumentation includes: two Becton Dickinson FACSAria flow cytometer cell sorters, a Bio-Rad S3e flow cytometer cell sorter, a Propel Labs Bigfoot Cell Sorter, a Bio-Rad ZE5 flow cytometer analyzer, an Agilent Novocyte Quanteon flow cytometer analyzer, and a Luminex ImageStreamX MkII imaging cytometer.
Duties include but are not limited to: instrument calibration, maintenance, and troubleshooting; instrument training for independent usage of the machines; assistance for users during instrument operation; and consultations with users regarding experimental design, data analysis, and troubleshooting. The candidate will demonstrate a high level of technical expertise relevant to the theory of flow cytometry, cell sorting, and imaging cytometry, the architecture of a flow cytometer as it relates to instrument operation and troubleshooting, and a level of knowledge of biology sufficient to assist users to successfully set up and execute flow cytometry experiments.
In particular, a significant component of the position will be to lead our effort to support an Amnis ImageStreamX MkII imaging cytometer into the core facility. These duties will include developing and maintaining standard operating procedures (SOPs) and policies required to properly and effectively implement this technology into core operations, assisting new users establish experiments, training users on independent operation of the instrument, troubleshoot instrument issues, providing basic data analysis training, and providing ongoing support for users of this technology.
Minimum Degree Required
Bachelor’s degree or equivalent and 4 years of experience.
At least one year of experience with operating flow cytometry instrumentation.
In addition, the following skills are required:
●Deep understanding of flow cytometry theory and instrumentation
●Familiarity with FACSDiva software platform
●Facility to learn new software platforms as new tools and instrumentation are integrated into the facility
●Ability to multitask user support, instrument operation, and troubleshooting
●Excellent communication and interpersonal skills
The laboratory of Dr. Chia-Wei Cheng, is seeking a motivated and research-oriented laboratory technician. Dr. Cheng's research team studies the metabolic cell-fate determinants that orchestrate tissue adaptation to diet. She believes the translation of nutrition facts into stem cell fate decisions will help inform dietary recommendations and inspire therapeutic strategies. Towards this end, Dr. Cheng’s laboratory is employing a variety of cross-disciplinary approaches using primarily genetic mouse models and organoid culture system. Current projects investigate the therapeutic potential of nutrient and metabolite signaling in treating cancer, diabetes and age-related degenerative diseases.
Reporting to the PI and working under the day-to-day direction of senior lab members, the Technician will help with the proper running of the laboratory and perform analyses related to a wide range of experiments both in a supervised and independent manner. This is an opportunity for the right candidate to strengthen his/her laboratory skills as part of a dynamic laboratory team at the forefront of stem cell research.
- Perform experiments under the direction of senior lab personnel, or conduct independently components of such experiments, which may include areas such as preclinical animal studies, tissue collection and dissection, cell/tissue/organoid culture and histologic examination and molecular analyses.
- Record and track experimental results accurately.
- Assist with genotyping and management of mouse colonies.
- Assist with ordering and maintaining inventory of laboratory supplies, perform assays.
- Prepare solutions adhering to specific instructions, and maintain equipment or specific laboratory resources.
- Bachelor's degree in biology, molecular biology, or biochemistry or Bachelor's degree in another field with relevant coursework, laboratory experience, demonstrated understanding of the fundamental concepts of biological sciences or a combination of education, training and experience.
- Successful completion of applicable compliance and system training requirements
- Have work or research experience in a biology lab.
- Must be detail-focused and organized. Must be able to take instructions and execute assignments working independently, seeking clarification and guidance where needed.
- Must demonstrate critical thinking skills and an aptitude for ongoing learning development, especially with regard to complex instrumentation and technology. For someone who demonstrates enthusiasm and aptitude for learning, this position offers the opportunity to strengthen laboratory and research skills, and build new ones.
- Excellent interpersonal skills and ability to adapt and work as part of a team. Strong communication skills, including the ability to ask questions and provide updates on work progress clearly and with sufficient technical detail.
- Solid computer skills.
Based in the Columbia Stem Cell Initiative, the full time Technician B will work in Dr. Joanna Smeeton's Laboratory at Columbia University Irving Medical Center. The lab studies musculoskeletal stem cell and developmental biology using zebrafish models.
The Technician B will perform various aspects of running, assisting with managing and writing results of basic experiments in molecular biology. This work will be primarily be performed under the supervision of the Principal Investigator but some independent work will be required.
The candidate will carry out molecular biology, biochemistry and cell biology on zebrafish, including molecular cloning, gene expression profiling, and live imaging with microscopes. In addition, the incumbent will manage zebra fish lines, purify DNA and RNA for the production of transgenic and mutant zebra fish, carry out microinjections, perform routine histological techniques including embedding and sectioning, keep organized laboratory records, maintain lab supplies, prepare solutions, administration duties to assist grant application. Any other duties as assigned.
Requires a bachelor's degree in chemistry, biology, physics or related science or equivalent combination of education, training and experience.
Willing to work some weekend hours. Highly organized with great attention to detail.
Excellent verbal and written communication skills, ability to prioritize, work independently and as a team member, exercise sound judgment and discretion, maintain a high level of confidentiality, and the ability to work under pressure, are essential.
Computer skills, including Photoshop, Illustrator, Excel and Word.
Strong organizational, interpersonal, analytical and research/writing skills.