We are looking for a highly motivated research assistant skilled in cell and tissue culture and basic laboratory techniques to join our lung regeneration project. Please see gvnlab.bme.columbia.edu for more information. The Research Assistant will support a broad range of research activities on lung regeneration. This is a grant-funded position and the annual continuation of the appointment is subject to review and funding. The position is available immediately.
CSCI Flow Cytometry is a shared resource providing labs across all Columbia University campuses access to high-end, well maintained flow cytometry, cell sorting, imaging cytometry, and other support instrumentation along with related support services including instrument training, assisted instrument operation, and experimental design, troubleshooting, and data analysis support. Cytometry instrumentation includes: two Becton Dickinson FACSAria flow cytometer cell sorters, a Bio-Rad S3e flow cytometer cell sorter, a Bigfoot Cell Sorter, a Bio-Rad ZE5 flow cytometer analyzer, a NovoCyte Quaneton flow cytometer analyzer, and a Luminex ImageStreamX MkII imaging cytometer.
The Flow Cytometry Technologist (Technician B) will support technical activities in the Columbia Stem Cell Initiative (CSCI) Flow Cytometry core facility.
Duties include but are not limited to:
- Instrument calibration, maintenance, and troubleshooting.
- Participation in genomics workflow that will be integrated in the core within the next year.
- Support of instrument training activities
- Performing user assisted sorts and analysis
- Basic sample preparation for instrument training
- Assistance with instrument and reagent testing
- Standard Operating Procedure (SOP) generation and review
- Lab maintenance duties including restocking and organization
Flow cytometry experience is not required but preferred if possible. This position is best fit for someone who is interested in technology’s role in supporting research in a busy shared resource environment. The candidate will be working directly with researchers and other staff members of the university in a dynamic environment, so excellent communication, interpersonal, and multitasking skills are required. The candidate will be working directly with complex instrumentation, so a level of comfort with highly-technical equipment is required.
Due to the role’s involvement in the 10x Genomics workflow, molecular biology experience, especially working with RNA, is highly desired.
This is an excellent opportunity to jump-start your career in the flow cytometry and genomics field in a well-run core facility.
This position requires a bachelor's degree in a scientific or engineering discipline OR demonstrated understanding of fundamental concepts in biology and basic laboratory skills.
There is currently an associate research scientist position available in the Stem Cell and Cellular Models Platform in the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain. The Platform, directed by Dr. Andrew Sproul, serves as a proactive resource to help generate human pluripotent stem cell (PSC) models of Alzheimer’s disease (AD) and other neurodegenerative diseases, with a large focus on late-onset AD (LOAD). LOAD risk factors, and other genomic modifications, will be knocked into control PSC backbones using CRISPR/Cas9 technology. These isogenic PSC lines will subsequently be differentiated into disease-relevant cell types in both monolayer and organoid formats for functional assays. Differentiation strategies include overexpression of transcription factors by lentiviral transduction. As these are very collaborative projects with other research groups within the Taub Institute, good communication skills are essential for this position. In addition, leadership and strong organizational skills are required, as the associate research scientist will help manage the day-to-day operations of the Platform and technicians, in coordination with the lab manager. While the primary focuses of the position are to assist with and help manage collaborative projects, as well as to help translate/develop new differentiation protocols, the associate research scientist will also lead more mechanistic studies driven by Dr. Sproul.
At least two years postdoctoral research experience with a strong cell culture background.
Preference will be given to candidates with stem cell, primary neuronal culture, or genome editing experience. Ideally the candidate will have some experience in all three approaches