The laboratory of Dr. Emmanuelle Passegué, Director of the Columbia Stem Cell Initiative and Principal Investigator (PI), is seeking an organized and energetic laboratory technician. Dr. Passegué’s research team investigates the cellular and molecular processes controlling the activity of blood-forming hematopoietic stem cells (HSC) during homeostasis and addresses how these regulations are altered in myeloid malignancies and physiological aging. The goal of Dr. Passegué’s research is to identify affected genes and/or pathways that can be used to develop new therapies to treat human diseases. Towards this end, the Passegué laboratory is employing a variety of cross-disciplinary approaches using primarily genetic mouse models. Current projects investigate the role of apoptosis, autophagy, immune regulations, DNA repair mechanisms, and changes in the bone marrow (BM) niche microenvironment in HSC function in normal and deregulated conditions.
Reporting to the PI and working under the day-to-day direction of the Lab Manager, the Technician will help with the proper running of the laboratory and perform analyses related to a wide range of experiments both in a supervised and independent manner. This is an opportunity for the right candidate to strengthen lab skills as part of a dynamic laboratory team at the forefront of stem cell research.
- Perform fully-planned experiments under the direction of senior lab personnel, or conduct independently components of such experiments, which may include areas such as genotyping, flow cytometry, transplantation experiments, cell culture, cell biology, molecular biology, confocal imaging, and histological analyses.
- Record and track experimental results accurately.
- Assist with management of mouse colonies.
- Assist with ordering and maintaining inventory of laboratory supplies, perform assays and prepare solutions adhering to specific instructions, and maintain equipment or specific laboratory resources.
Bachelor's degree in a cell biology, molecular biology, or biochemistry or Bachelor’s degree in another field with relevant coursework, laboratory experience, and a demonstrated understanding of the fundamental concepts of biological sciences or a combination of education, training and experience.
- Must be detail-focused and organized. Must be able to take instructions and execute assignments working independently, seeking clarification and guidance where needed.
- Must demonstrate critical thinking skills and an aptitude for ongoing learning development, especially with regard to complex instrumentation and technology. For someone who demonstrates enthusiasm and aptitude for learning, this position offers the opportunity to strengthen laboratory and research skills, and build new ones.
- Excellent interpersonal skills and ability to adapt. Strong communication skills, including the ability to ask questions and provide updates on work progress clearly and with sufficient technical detail.
- Solid computer skills.
The Technician Assistant is responsible for performing routine but important maintenance tasks within the Columbia Stem Cell Initiative’s laboratories. The position reports to the Laboratory Manager and also works closely with the Executive Assistant to the Director.
- Pick up, wash, sterilize, and redistribute glassware and plastic ware for research laboratories
- Prepare glass and plastic laboratory supplies for use by lab staff
- Restock supplies from inventory
- Sterilize solutions for individual labs and common solutions
- Ensure laboratory space and common areas are maintained in a neat and orderly condition; report to department any conditions requiring maintenance or custodial service
- Pick up/drop off correspondence from/to institutional offices as required
- Post lab/departmental seminar flyers and similar announcements in designated locations when supplied by event organizer
- Assist with seating arrangements for seminars and lab meetings as needed
- Identify backup glassware technician assistant in anticipation of absences
- Perform other lab maintenance and assistant duties as assigned
High School graduate or equivalency certificate. Technical training or equivalency and some related laboratory experience, usually six months
Experience working in a research laboratory is desirable
- Ability to use autoclave and dish washing equipment
- Perform other lab maintenance and assistant duties as assigned
- Ability to lift supplies and push/pull items on cart
- Ability to travel around campus and hang/distribute materials
- Strong interpersonal and communication skills, including the aptitude to ask questions and provide updates on work progress in person and by email
Research technician is available in the Department of Genetics & Development at Columbia University Medical Center in New York in the laboratory of Dr. Vincenzo A. Gennarino. We are a multidisciplinary lab working with cell biology, RNA biology, biochemistry, general molecular biology, and transgenic/knockout mouse models. The successful candidate would be responsible for providing technical support for all projects running in the laboratory. This would include overseeing day-to-day operations, maintaining supplies and servicing equipment but also developing and optimizing new protocols and training new laboratory members. Additionally to the technical support, the successful candidate would have an opportunity to carry out an own research project within available capacity. Experience in mouse work would be advantageous.
Applicants should send cover letter, curriculum vitae and names and addresses of three references by email to: Vincenzo A. Gennarino, Ph.D.: email@example.com
There is currently an Associate Research Scientist position available in the Stem Cell and Cellular Models Platform in the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain. The Platform, directed by Dr. Andrew Sproul, serves as a proactive resource to help generate human pluripotent stem cell (PSC) models of Alzheimer’s disease (AD) and other neurodegenerative diseases, with a large focus on late-onset AD (LOAD). LOAD risk factors, and other genomic modifications, will be knocked into control PSC backbones using CRISPR/Cas9 technology. These isogenic PSC lines will subsequently be differentiated into disease-relevant cell types in both monolayer and organoid formats for functional assays. Differentiation strategies include overexpression of transcription factors by lentiviral transduction. As these are very collaborative projects with other research groups within the Taub Institute, good communication skills are essential for this position. In addition, leadership and strong organizational skills are required, as the associate research scientist will help manage the day-to-day operations of the Platform and technicians, in coordination with the lab manager. While the primary focuses of the position are to assist with and help manage collaborative projects, as well as to help translate/develop new differentiation protocols, the associate research scientist may assist with more mechanistic studies driven by Dr. Sproul if funding permits.