The Molecular Imaging and Neuropathology is looking to hire an Assistant Research Scientist (MS preferred) to work with the PI on immunohistochemistry and in situ hybridization experiments in human brain tissue
The candidate will be conducting studies involving the use of immunohistochemistry and stereology, in situ hybridization, western blotting and film imaging and quantification on human brain tissue samples, and iPSC-derived neural stem cells and their neuronal differentiation. These procedures are directed at the study of neurobiological aspects of neuropsychiatric diseases. The position requires learning and understanding the human brain anatomy, determining cell numbers, density, and morphometry and autoradiography quantification.
Specific duties include:
The duties will include: in situ hybridization assays and autoradiography; immunohistochemistry, operation of general laboratory instruments, keep inventory of freezer contents, bright field, fluorescence and confocal microscopy, stereology, perform data management, data reporting using graphs and images, literature search and generating bibliography for papers and posters. Additional duties include operation of the computer image analysis software Neurolucida, Stereo Investigator, and Densita, with the objective of data collection and analysis by providing Image Analysis support and assistance to the Principal Investigator, and creating graphics (charts, graphs, plotting, spreadsheet evaluation).
Bachelor or Master degree in Biology or related field, strong computer skills including Word, Excel, PowerPoint are required.
Some years of experience with immunohistochemistry, Endnote, Confocal Microscopy, Neurolucida, Stereoinvestigator and Densita are required. Experience in data reporting using graphs and images, literature search and generating bibliography for papers and posters are required.
Preferred skills in cell cultures and iPSC studies, preferred experience with tissue clearing techniques and 3D imaging software, preferred experience with statistical software.
Master degree preferred. Applicants are also expected to have excellent organizational skills, keep detailed records, and collaborate with others in the laboratory with data management. She/he should be able to work on a flexible schedule, including some weekends and evenings.
The Research Foundation is a private not-for-profit corporation and is not an agency or instrumentality of the State of New York. Employees of the Research Foundation are not state employees, do not participate in any state retirement system, and do not receive state fringe benefits. Excellent Benefits Package. Affirmative Action/Equal Opportunity Employer/Minority/Women/Disabled/Veteran Employer. VEVRAA 41 CFR 60-300.5(a) compliant.
Applications will only be accepted through website at: http://corporate.rfmh.org then click on Employment Opportunities. Applications will be accepted only during the posting dates as listed at the top of the job announcement. Contact information: firstname.lastname@example.org
The laboratory of Dr. Emmanuelle Passegué, Director of the Columbia Stem Cell Initiative and Principal Investigator (PI), Is seeking an organized and energetic laboratory technician. Dr. Passegué's research team investigates the cellular and molecular processes controlling the activity of blood-forming hematopoietic stem cells (HSC) during homeostasis and addresses how these regulations are altered in myeloid malignancies and physiological aging. The goal of Dr. Passegue's research is to identify affected genes and/or pathways that can be used to develop new therapies to treat human diseases. Towards this end, the Passegue laboratory is employing a variety of cross-disciplinary approaches using primarily genetic mouse models. Current projects investigate the role of apoptosis, autophagy, immune regulations, DNA repair mechanisms, and changes in the bone marrow (BM) niche microenvironment In HSC function in normal and deregulated conditions.
Reporting to the PI and working under the day-to-day direction of the Lab Manager or senior lab members, the Technician will help with the proper running of the laboratory and perform analyses related to a wide range of experiments both in a supervised and independent manner. This is an opportunity for the right candidate to strengthen his/her laboratory skills as part of a dynamic laboratory team at the forefront of stem cell research.
• Perform fully-planned experiments under the direction of senior lab personnel, or conduct independently components of such experiments, which may include areas such as genotyping, flow cytometry, transplantation experiments, cell culture, cell biology, molecular biology, confocal imaging, and histological analyses.
• Record and track experimental results accurately.
• Assist with management of mouse colonies.
• Assist with ordering and maintaining inventory of laboratory supplies, perform assays and prepare solutions adhering to specific instructions, and maintain equipment or specific laboratory resources.
• Bachelor's degree in biology, molecular biology, or biochemistry or Bachelor's degree in another field with relevant coursework, laboratory experience, demonstrated understanding of the fundamental concepts of biological sciences or a combination of education, training and experience.
• Must be detail-focused and organized. Must be able to take instructions and execute assignments working independently, seeking clarification and guidance where needed.
• Must demonstrate critical thinking skills and an aptitude for ongoing learning development, especially with regard to complex instrumentation and technology. For someone who demonstrates enthusiasm and aptitude for learning, this position offers the opportunity to strengthen laboratory and research skills, and build new ones.
• Excellent interpersonal skills and ability to adapt and work as part of a team. Strong communication skills, including the ability to ask questions and provide updates on work progress clearly and with sufficient technical detail.
• Solid computer skills.