Staff Opportunities

Flow Cytometrist (Technician B)- CSCI Flow Cytometry Core

The Columbia Stem Cell Initiative (CSCI) Flow Cytometry core facility is a busy shared instrument resource that provides Columbia University laboratories access to high-end, state-of-the-art flow cytometry instrumentation and services. CSCI Flow Cytometry currently maintains four cell sorters, three cell analyzers, and an imaging cytometer as well a real-time PCR and multiplexed cytokine array instrumentation. In addition, the core facility offers access to 10x Genomics single-cell capture technology. Our services utilized by over 300 researchers from over 150 individual labs from a diverse array of research areas, including stem cell biology, immunology, cancer biology, and neuroscience.

We are currently seeking a motivated candidate to join the CSCI's growing team as a Flow Cytometrist (Technician B). This is an exciting opportunity to be a part of a critical research resource, acquire expertise in extremely technically complex instrumentation and be a part of cutting-edge science at  the top-tier Columbia University Medical Center through interaction with researchers from a wide range of research areas. Although previous experience with flow cytometry instrumentation is a plus, it is not required. We will provide thorough training for the right candidate who demonstrates motivation, eagerness, and an aptitude for learning. This is an opportunity to join and develop career skills in a growing field. 

Primary Responsibilities

  • Perform flow cytometer and cell sorter daily setup, calibration, and routine maintenance
  • Operate instrument for Columbia University researchers
  • Train users to independently operate cell sorters, cell analyzers and other equipment
  •  Perform routine instrument troubleshooting, intervention, and reporting
  • Communicate with instrument manufacturers for coordination of timely repairs
  • Assist with the development of standard operating procedures (SOPs) and training materials
  • Participate in research and development projects as time permits

Please note that this position may require some flexibility of working hours, including occasional required overtime and occasional opportunity to earn comp time. 

CSCI Flow Cytometry is an extremely busy laboratory serving the needs of many researchers in a single day. Success in this position requires independently learning, an aptitude for troubleshooting, the ability to multitask and excellent interpersonal skills.

Minimum Qualifications

  • Bachelor's degree in cell biology, molecular biology, or biochemistry OR Bachelor's degree in another field with relevant coursework, laboratory experience, and a demonstrated understanding of the fundamental concepts of biological sciences.
  • Independent and critical thinking skills. Successful instrument troubleshooting, which is a major component of this position, requires implementing a logical workflow, based on knowledge of flow cytometry principles and instrument components, to discover root causes and resolve issues.
  • Aptitude for constant learning and development, especially with regard to complex instrumentation and technology.
  • Excellent interpersonal skills and ability to adapt. This position requires constant interaction with a large user base of varying experience, so patience, willingness to teach, and ability to resolve conflicts in an assertive, professional, and effective manner is critical for success.
  • Facility with computers and the ability to adopt new software platforms

Preferred Qualifications

  • Knowledge of the principles of flow cytometry  
  • Experience performing flow cytometry assays
  • Prior experience with flow cytometry instrumentation
  • Knowledge of flow cytometry data analysis
  • Desktop support or other professional computer skills 

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Staff Associate/Lab Manager- Passegue Lab

Seeking an outstanding Staff Associate/Lab Manager with expertise in mouse experiments, flow cytometry or genomic analyses to study the cellular and molecular processes controlling the activity of blood-forming hematopoietic stem cells (HSC) during homeostasis, stress, disease and aging. This position is in the laboratory of Dr. Emmanuelle Passegué, Director of the Columbia Stem Cell Initiative and Professor in the Department of Genetics and Development at Columbia University Irving Medical Center. Reporting to the PI and serving as laboratory manager, the candidate will be an active member of the research team helping or driving independent research projects. The candidate will also supervise and coordinate laboratory operation and protocols to ensure optimal performance of personnel, equipment and utilization of space and supplies. This is an opportunity to join a dynamic laboratory at the forefront of stem cell research.

Preferred qualifications:

  • Experience with independent scientific research to execute experiments and research tasks, and help to plan future research strategy. Scientific growth will be encouraged, and research projects will be assigned as needed.
  • Experience with maintaining mouse colonies, genotyping, flow cytometry, transplantation experiments, cell culture, cell biology, molecular biology, confocal imaging, histological analyses, etc.
  • Experience with ordering and managing equipment and in overseeing protocols, maintaining records, managing databases, and working closely with administrative and financial personnel.
  • Ability to work independently and as part of a team to train and assist other lab members.
  • Excellent interpersonal skills and written/oral communication skills.
  • Solid computer skills.

Education: Bachelor’s degree in biochemistry, genetics, developmental biology, biomedical engineering, or an equivalent, and at least 4 years of related experience. Master’s degree strongly preferred.

Position salary is competitive and will be based on qualifications.

Applicants should send cover letter, curriculum vitae, and contact information for three references to:

Lalena Luna, csci-admin@cumc.columbia.edu

Executive Assistant to Dr. Emmanuelle Passegué

Apply Here

Staff member/Technician B- Passegue Lab

Seeking an outstanding, organized and energetic staff member/technician B to contribute to studies investigating the cellular and molecular processes controlling the activity of blood-forming hematopoietic stem cells (HSC) during homeostasis, stress, disease and aging. This position is in the laboratory of Dr. Emmanuelle Passegué, Director of the Columbia Stem Cell Initiative and Professor in the Department of Genetics and Development at Columbia University Medical Center. Reporting to the PI and working under the day-to-day supervision of senior lab members, the candidate will be involved in a wide range of experiments both in a supervised and independent manner, and will contribute to the proper running of the laboratory. This is an opportunity to join a dynamic laboratory at the forefront of stem cell research.

Preferred qualifications:

  • Will perform planned experiments under the direction of senior lab personnel, or conduct independently components of such experiments, which may include areas such as genotyping, flow cytometry, transplantation experiments, cell culture, cell biology, molecular biology, confocal imaging, and histological analyses.
  • Must be detail-focused and organized. Must be able to take instructions and execute assignments working independently, seeking clarification and guidance where needed.
  • Must demonstrate critical thinking skills and an aptitude for ongoing learning development, especially with regard to complex instrumentation and technology. For someone who demonstrates enthusiasm and aptitude for learning, this position offers the opportunity to strengthen laboratory and research skills, and build new ones.
  • Excellent interpersonal skills and ability to adapt and work as part of a team. Strong communication skills, including the ability to ask questions and provide updates on work progress clearly and with sufficient technical detail.
  • Solid computer skills.

Education: Bachelor's degree in biology, molecular biology, or biochemistry or Bachelor's degree in another field with relevant coursework, laboratory experience, demonstrated understanding of the fundamental concepts of biological sciences or a combination of education, training and experience.

Apply Here

Applicants should also send cover letter, curriculum vitae, and contact information for three references to:

Lalena Luna, csci-admin@cumc.columbia.edu

Executive Assistant to Dr. Emmanuelle Passegué

 

Associate Research Scientist- Sproul Lab, Taub Institute

There is currently an associate research scientist position available in the Stem Cell and Cellular Models Platform in the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain. The Platform, directed by Dr. Andrew Sproul, serves as a proactive resource to help generate human pluripotent stem cell (PSC) models of Alzheimer’s disease (AD) and other neurodegenerative diseases, with a large focus on late-onset AD (LOAD). LOAD risk factors, and other genomic modifications, will be knocked into control PSC backbones using CRISPR/Cas9 technology. These isogenic PSC lines will subsequently be differentiated into disease-relevant cell types in both monolayer and organoid formats for functional assays. Differentiation strategies include overexpression of transcription factors by lentiviral transduction. As these are very collaborative projects with other research groups within the Taub Institute, good communication skills are essential for this position. In addition, leadership and strong organizational skills are required, as the associate research scientist will help manage the day-to-day operations of the Platform and technicians, in coordination with the lab manager. While the primary focuses of the position are to assist with and help manage collaborative projects, as well as to help translate/develop new differentiation protocols, the associate research scientist will also lead more mechanistic studies driven by Dr. Sproul.

Responsibilities

  • Conduct research in an independent manner
  • Lead multiple collaborative projects in parallel
  • Assist in running the Platform by helping to supervise and guide technicians and research assistants
  • Assist in manuscript and grant writing

Minimum Qualifications

At least two years postdoctoral research experience with a strong cell culture background.

Preferred Qualifications

Preference will be given to candidates with stem cell, primary neuronal culture, or genome editing experience. Ideally the candidate will have some experience in all three approaches.

Apply here

 

Technician B- Sproul Lab, Taub Institute

A Technician B position is available in Dr. Andrew Sproul’s lab in the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain at Columbia University Medical Center. Dr. Sproul directs the Stem Cell and Cellular Models Platform, a rapidly evolving resource for Taub researchers that creates stem cell models of Alzheimer’s disease and other neurodegenerative and neurological disorders. Duties will include significant cell culture work as well as assays such as immunocytochemistry, PCR, lentivirus production, and DNA/RNA isolation among other molecular techniques. Other responsibilities will include maintaining lab equipment and supplies. The applicant must be able to multitask effectively with different projects and have strong organizational skills. The ideal candidate will be engaged in the scientific questions being investigated as well as proactive in helping to maintain an organized and effective laboratory environment.

Responsibilities

  • Maintain stem cell cultures and conduct differentiation experiments.
  • Genome-edit stem cell lines using CRISPR/Cas9 technology.
  • Conduct molecular biology assays: i.e. DNA/RNA prep, PCR, immunostaining, etc.
  • Assist with tracking of laboratory supplies, general lab organization.

Minimum Qualifications

Bachelor degree in biology, chemistry, physics, or equivalent in education, training, and experience.

Preferred Qualifications

  • Previous cell culture experience is strongly preferred.
  • Some molecular biology experience is preferred.
  • Prior technical or undergraduate research experience is preferred.

Apply Here

Assistant Research Scientist- Boldrini Lab

The Molecular Imaging and Neuropathology is looking to hire an Assistant Research Scientist (MS preferred) to work with the PI on immunohistochemistry and in situ hybridization experiments in human brain tissue.The candidate will be conducting studies involving the use of RNA and ATAC sequencing, multiplex immunofluorescence and immunohistochemistry, RNAscope® in situ hybridization, and western blotting.  Imaging lab will involve brain tissue imaging and quantification, confocal microscopy and stereology using MBF Inc. Stereoinvestigator and Neurolucida. These techniques are directed at the study of neurobiological aspects of neurodegenerative and psychiatric illness. The position requires understanding human brain anatomy, attention to detail, fast learning abilities, experience in wet and imaging lab, data managing and excellent organizational and team building skills.

  • The duties will include preparing cell and tissue for single nucleus and slide-mounted spatial RNA and ATAC sequencing, performing multiplex immunofluorescence and immunohistochemistry, RNAscope® in situ hybridization, and western blotting.
  • The position involves teaching opportunities, working with graduate and undergraduate students, and collaboration with postdocs.
  • Imaging lab will involve brain tissue imaging and quantification, confocal microscopy and stereology using MBF Inc. Stereoinvestigator and Neurolucida.
  • Other tasks will involve operation of cryostat and microtome, keeping inventory of freezer contents, lab supplies, and brain information. The RA will perform data reporting and presentations, and literature search for papers and posters, and be involved in manuscript writing and poster presentations.
  • The RA will meet daily with the PI and participate in the weekly lab meetings.

Minimum Qualifications

Bachelor or Master degree with Major in Neuroscience, Biology, or related field, strong computer skills including Word, Excel, PowerPoint. Experience in immunohistochemistry, immunofluorescence, confocal microscopy, data reporting using graphs and images. Excellent writing skills, communication skills and teamwork attitude are required.

Preferred Qualifications

Master degree, experience with R, Python, SAS, SPSS, Neurolucida and Stereoinvestigator software, manuscripts writing preferred.

 

Apply Here