The Molecular Imaging and Neuropathology is looking to hire an Assistant Research Scientist (MS preferred) to work with the PI on immunohistochemistry and in situ hybridization experiments in human brain tissue.The candidate will be conducting studies involving the use of RNA and ATAC sequencing, multiplex immunofluorescence and immunohistochemistry, RNAscope® in situ hybridization, and western blotting. Imaging lab will involve brain tissue imaging and quantification, confocal microscopy and stereology using MBF Inc. Stereoinvestigator and Neurolucida. These techniques are directed at the study of neurobiological aspects of neurodegenerative and psychiatric illness. The position requires understanding human brain anatomy, attention to detail, fast learning abilities, experience in wet and imaging lab, data managing and excellent organizational and team building skills.
- The duties will include preparing cell and tissue for single nucleus and slide-mounted spatial RNA and ATAC sequencing, performing multiplex immunofluorescence and immunohistochemistry, RNAscope® in situ hybridization, and western blotting.
- The position involves teaching opportunities, working with graduate and undergraduate students, and collaboration with postdocs.
- Imaging lab will involve brain tissue imaging and quantification, confocal microscopy and stereology using MBF Inc. Stereoinvestigator and Neurolucida.
- Other tasks will involve operation of cryostat and microtome, keeping inventory of freezer contents, lab supplies, and brain information. The RA will perform data reporting and presentations, and literature search for papers and posters, and be involved in manuscript writing and poster presentations.
- The RA will meet daily with the PI and participate in the weekly lab meetings.
Bachelor or Master degree with Major in Neuroscience, Biology, or related field, strong computer skills including Word, Excel, PowerPoint. Experience in immunohistochemistry, immunofluorescence, confocal microscopy, data reporting using graphs and images. Excellent writing skills, communication skills and teamwork attitude are required.
Master degree, experience with R, Python, SAS, SPSS, Neurolucida and Stereoinvestigator software, manuscripts writing preferred.
We are looking for a highly motivated research assistant skilled in cell and tissue culture and basic laboratory techniques to join our lung regeneration project. Please see gvnlab.bme.columbia.edu for more information. The Research Assistant will support a broad range of research activities on lung regeneration. This is a grant-funded position and the annual continuation of the appointment is subject to review and funding. The position is available immediately.
There is currently an associate research scientist position available in the Stem Cell and Cellular Models Platform in the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain. The Platform, directed by Dr. Andrew Sproul, serves as a proactive resource to help generate human pluripotent stem cell (PSC) models of Alzheimer’s disease (AD) and other neurodegenerative diseases, with a large focus on late-onset AD (LOAD). LOAD risk factors, and other genomic modifications, will be knocked into control PSC backbones using CRISPR/Cas9 technology. These isogenic PSC lines will subsequently be differentiated into disease-relevant cell types in both monolayer and organoid formats for functional assays. Differentiation strategies include overexpression of transcription factors by lentiviral transduction. As these are very collaborative projects with other research groups within the Taub Institute, good communication skills are essential for this position. In addition, leadership and strong organizational skills are required, as the associate research scientist will help manage the day-to-day operations of the Platform and technicians, in coordination with the lab manager. While the primary focuses of the position are to assist with and help manage collaborative projects, as well as to help translate/develop new differentiation protocols, the associate research scientist will also lead more mechanistic studies driven by Dr. Sproul.
At least two years postdoctoral research experience with a strong cell culture background.
Preference will be given to candidates with stem cell, primary neuronal culture, or genome editing experience. Ideally the candidate will have some experience in all three approaches